Journal: Herz
Article Title: Tetrandrine has protective role in myocardial ischemia/reperfusion injury via the TRPV2/Ca 2+ /calcineurin/NFAT axis
doi: 10.1007/s00059-025-05334-w
Figure Lengend Snippet: TET downregulates TRPV2 expression and inhibits the Ca 2+ /calcineurin/NFAT pathway. a mRNA expression and b protein levels of TRPV2, calcineurin, and NFAT in H/R-challenged H9c2 cells after treatment with different concentrations (0.5, 1.0, and 1.5 μM) of TET as measured by qRT-PCR and western blot, respectively. c, d ROS amounts in H/R-challenged H9c2 cells after treatment with different concentrations (0.5, 1.0, and 1.5 μM) of TET as calculated by DCFH-DA staining. Scale bar = 100 μm. e Ca 2+ concentrations in H/R-challenged H9c2 cells treated with different concentrations (0.5, 1.0, and 1.5 μM) of TET as determined using a calcium assay kit. Double asterisk: p < 0.01, triple asterisk: p < 0.001, quadruple asterisk: p < 0.0001 vs. control; diamond: p < 0.05, double diamond: p < 0.01, triple diamond: p < 0.001, quadruple diamond: p < 0.0001 vs. H/R
Article Snippet: Calcineurin , 16569-1-AP , 1:1000 , Proteintech, Wuhan, China.
Techniques: Expressing, Quantitative RT-PCR, Western Blot, Staining, Calcium Assay, Control